Biochemical characterization of mutants generated by sodium azide treatment of corresponding sensitive wild Actinomycete strains isolated from Khumbu, Everest Base Camp
Here goes below an abstract of work of Mamata Khatri and Yurika Rajbhandari who pursued their thesis conducted under my supervision and Prof. Agrawal as a partial fulfillment of their B. Sc. Biochemistry degree from Universal Science College, Pokhara University. They jointly worked to characterize biochemically the actinomycete mutants generated by sodium azide treatment of corresponding sensitive wild strains isolated from Khumbu, Everest Base Camp. The work is granted by International Foundation for Science (IFS) – 2008. This endeavor followed the findings of previous student, Keshav Bhattarai for his thesis work. With his work, we discovered various Gain-of-Function and Loss-of-Function mutations in a Streptomyces strain isolated from a soil sampele from Everest Base Camp.
Abstract: Sodium azide is being used to generate mutants of Actinomycetes isolated in Research Laboratory for Biotechnology and Biochemistry (RLABB) from soil samples from Khumbu, Mount Everest Base camp. This work was done to expand the limited number of actinomycete mutants sensitive 10-50ppm of sodium azide and characterize them biochemically alongwith the corresponding wild strains. Actinomycetes were isolated in Starch Casein Agar (SCA) from the soil samples and subsequently purified. Of the total 36 strains from RLABB, 24 were found to be sensitive to 50 – 100 ppm of sodium azide and were selected to generate mutants. Based on differences in colonial characteristics compared to the wild strains, 32 of mutants were selected and purified in an agar medium without the mutagen. The mutants and corresponding wild types were characterized biochemically and observed various Gain-of-Function (GOF) and Loss-of-function (LOF) mutation.
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